special ecological reason for the presence of agarolytic bacteria in fresh water but that Identification of Medical Bacteria, 2nd edn. Cam-. Endolytic β-agarase Aga2 was identified from Cellulophaga omnivescoria W5C. SJP92 was shown to retain almost 90% of agarolytic activity under Recently, thermostable agarases from marine bacteria Flammeovirga sp. Abstract: Agarolytic bacteria use agarase to utilize agar as sole source of carbon. It is usually observed in life sciences labs that lot of agar medium needs to be.

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Isolation And Identification of agarolytic bacteria in Marin by Faizah Rahman on Prezi

Agar clearing, softening, and depressions around the colonies is characteristic for bacteria in groups 1 and 2. Van der Meulen H, Vedkamp H. Chromatography of agarase from P. Agarase N-1 had a molecular mass of 33 kDa, as determined by a comparison with the mobility of protein standards Fig.

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Strain N-1 and P. However, we must point out that strain N-1 differs from the type strain of this species in some properties. This possibility seems feasible because the enzyme could not be eluted from agarose columns as it is on other agarases 3.


The oligosaccharides were detected by evaluation of the refractive index. A Effect of pH on the activity of the purified agarase. Strain N-1 was cultured in liquid medium containing 0. Some of the bacterial isolates have been assigned to the genera Alteromonas 1221 idengification, 2733Cytophaga 43Streptomyces 36Vibrio 339and Pseudomonas The genera Alteromonas and Marinomonas.

Support Center Support Center. The enzyme was slowly released from the DEAE-cellulose by a washing with 1. The agarase gene dagA of Streptomyces coelicolor A3 2: DNA base composition of Rickettsia tsutsugamushi determined by reversed-phase high-performance liquid chromatography. The enzyme was concentrated with polyethylene glycol and dialyzed against buffer A.

In solid agar, this bacterria produced a diffusible agarase that caused agar softening around the colonies. Purification and properties of an extracellular agarase from Alteromonas sp. Please review our privacy policy. Strain N-1 is a gram-negative rod bacterium, motile by a polar flagellum; it is also obligate aerobic, catalase and oxidase positive, and urease, indole, and arginine dihydrolase negative.

Manual of methods for general microbiology. Phylogenetic analysis and assessment of the genera VibrioPhotobacterium bavteria, and Plesiomonas deduced from small-subunit rRNA sequences. The rDNA sequence of strain N-1 was compared to sequences available from public databases.

Taxonomy of aerobic marine eubacteria. Furthermore, the reducing power of the agarooligosaccharides is greatly decreased by the presence of 3,6-anhydro- l -galactose at the reducing end Effects of pH and temperature on enzyme activity. American Society for Microbiology; Strain N-1 was isolated from decomposing algae in Niebla Valdivia, Chile. Phylogeny of the Vibrionaceae and recommendation for two new genera, Listonella and Shewanella.


Utilization of N -acetylglucosamine, cellobiose, d -fructose, d -galactose, d -glucose, glycogen, inulin, lactose, maltose, d -mannose, mannitol, sacarose, and d -xylose.

In both cases the enzyme showed a molecular mass of 16 kDa, indicating an interaction with these resins. B Effect of temperature on the oc of agarase from P.

Fractions 3 ml were collected, pooled on the basis of activity, and then loaded afarolytic the DEAE-cellulose column 10 by 1. The exception is Alteromonas sp. Other biochemical and physiological tests were carried out essentially as described by Stolp and Gadkari 38 and Stanier et al.

Strain N-1 can be distinguished from P. It requires sodium ion for growth, has an oxidative metabolism, and does not accumulate polyhydroxybutyrate as an intracellular reserve. Previous studies have shown that agar degradation can occur by two mechanisms that depend on the specificity of the cleaving enzymes.